Based at the Monash Health Translation Precinct, the Functional Genomics Capability provides the tools for unbiased, systematic, high-throughput, genome-wide, gain-of-function and loss-of-function studies in human and mouse cells.
We offer pooled library screens, using CRISPR-Cas9 technology (CRISPRc, CRISPRi, CRISPRa) and ORF libraries, to support investigations ranging from fundamental biology through to addressing clinical need.
We work closely with investigators and deliver comprehensive project management, based upon fee-for-service model, including
- Project design and implementation strategy
- Genome wide or smaller custom designed pooled sgRNA libraries
- Creation of Cas9/dCas9 cells for CRISPR screens
- Pooled library screening and selection
- Massively parallel sequencing (next generation sequencing)
- Bioinformatic analysis and data visualization
- Secondary screening, for example single cell transcriptomics (Dolomite Bio Single Cell RNA-Seq)
- Bioinformatic analysis and mechanistic characterization of secondary screening hits.
A/Prof Joseph (Sefi) Rosenbluh,
MHTP Functional Genomics Capability Director
t: +61 3 9902 9257
Dr Luis Malaver-Ortega,
MHTP Functional Genomics Capability Manager
t: +61 3 8572 2458